Saint Helena's Parish was organized in 1712 and construction of the church began in 1724. Colonel Jack "Tuscarora Jack" Barnwell founder of Beaufort, is buried here.
The church was used as a hospital during the Civil War, when its flat tombstones became operating tables. The church is open daily, September through May from 10:00 a.m. until 4:00 p.m., and June through August from 10:00 a.m. until 1:00 p.m.